| Info: | Assignments in education
Dr. Holzer accompanies the following courses within the Department of Pharmaceutical Technology: - Praktikum "Pharmazeutische Technologie" (STEX)
- Praktikum "Physikalisch-chemische Übungen" (STEX/BSC)
- Seminar "Biopharmazie einschließlich arzneiformenbezogener Pharmakokinetik" (STEX/BSC)
- Seminar & Praktikum "Analytik, physikalische Methoden und Qualitätssicherung bei der Herstellung von Arzneimitteln" (BSC)
- Seminar & Praktikum "Ausgewählte Aspekte der Pharmazeutischen Technologie" (MSC)
Scientific methods
- Asymmetrical flow field-flow fractionation (AF4) with multiangle-light-scattering-, UV/VIS- and fluorescence detectors
- Free-flow electrophoresis
- Dynamic light scattering
- Zeta potential measurements
- High-performance thin layer chromatography for lipid analysis
- Size exclusion chromatography
- HPLC with corona charged aerosol detector
- Ultracentrifugation
Scientific publications
- Deuringer, B.; Härdtner, C.; Krebs, K.; Thomann, R.; Holzer, M.; Hilgendorf, I.; Süss, R.: Everolimus-loaded reconstituted high-density lipoprotein prepared by a novel dual centrifugation approach for anti-atherosclerotic therapy; Int. J. Nanomed. 2022: 17: 5081-5097 (2022). dx.doi.org/10.2147/IJN.S381483
- Langer, C.; Köll-Weber, M.; Holzer, M.; Hantel, C.; Süss, R.: Mitotane nanocarriers for the treatment of adrenocortical carcinoma: Evaluation of albumin-stabilized nanoparticles and liposomes in a preclinical in vitro study with 3D spheroids; Pharmaceuticals, 14: 1891 (2022). dx.doi.org/10.3390/pharmaceutics14091891
- Hurth, Z.; Faber, M.-L.; Gendrisch, F.; Holzer, M.; Haarhaus, B.; Cawelius, A.; Schwabe, K.; Schempp, C.M.; Wölfle, U.: The anti-inflammatory effect of Humulus lupulus extract in vivo depends on the galenic system of the topical formulation; Pharmaceuticals, 15: 350 (2022). dx.doi.org/10.3390/ph15030350
- Haizhang, C.; Maul-Pavicic, A.; Holzer, M.; Huber, M.; Salzer, U.; Chevalier, N.; Voll, R.E.; Hengel, H.; Kolb, P.: Detection and functional resolution of soluble immune complexes by an FcɣR reporter cell panel; EMBO Mol. Med.: e14182 (2021). dx.doi.org/10.15252/emmm.202114182
- De Souza, T.P.; Holzer, M.; Stano, P.; Steiniger, F.; May, S.; Schubert, R.; Fahr, A.; Luisi, P.L.: New insights into the growth and transformation of vesicles: a free-flow electrophoresis study; J. Phys. Chem. B 119: 12212-12223 (2015). dx.doi.org/10.1021/acs.jpcb.5b05057
- Vögtle, F.N.; Keller, M.; Taskin, A.T.; Horvath, S.E.; Guan, X.L.; Prinz, C.; Opalinska, M.; Zorzin, C., van der Laan, M.; Wenk, M.R.; Schubert, R.; Holzer, M.; Meisinger, C.: The fusogenic lipid phosphatidic acid promotes the biogenesis of mitochondrial outer membrane protein Ugo1; J. Cell Biol. 210: 951-960 (2015). dx.doi.org/10.1083/jcb.201506085
- Holzer, M.: Fiedler - Encyclopedia of Excipients. Monthly monograph updates 2013-today; WVG Stuttgart; www.drugbase.de/databases/fiedler.html
- Molnár, E.; Swamy, M.; Holzer, M.; Beck-García, K.; Worch, R.; Thiele, C.; Guigas, G.; Boye, K.; Luescher, I.F.; Schwille, P.; Schubert, R.; Schamel, W.A.: Cholesterol and sphingomyelin drive ligand-dependent T-cell antigen receptor nanoclustering; J. Biol. Chem. 287: 42664-42674 (2012). dx.doi.org/10.1074/jbc.M112.386045
- Holzer, M.; Momm, J.; Schubert R.: Lipid transfer mediated by a recombinant pro-sterol carrier protein 2 for the preparation of asymmetrical membrane vesicles requires a narrow vesicle size distribution: a FFE study; Langmuir 26: 4142-4252 (2010). dx.doi.org/10.1021/la903386d
- Holzer, M.; Burghardt, A.; Schubert, R.: Quantitative high-performance thin layer chromatography determination of liposome components and critical factors influencing the analysis results; J. Liposome Res. 20: 124-133 (2010). dx.doi.org/10.3109/08982100903218884
- Schneider, S.; Lenz, D.; Holzer, M.; Palme, K.; Süss, R.: Intracellular FRET analysis of lipid/DNA complexes using flow cytometry and fluorescence imaging techniques; J. Control. Rel. 145: 289-296 (2010). dx.doi.org/10.1016/j.jconrel.2010.04.016
- Holzer, M.; Barnert, S.; Momm, J.; Schubert, R.: Preparative size exclusion chromatography combined with detergent removal as a versatile tool to prepare unilamellar and spherical liposomes of highly uniform size distribution; J. Chrom. A 1216: 5838-5348 (2009). dx.doi.org/10.1016/j.chroma.2009.06.023
- Holzer, M.: Proteinvermittelter Lipidtransfer zwischen Liposomen zur Herstellung asymmetrischer Modellmembranen und die Rolle der Membranlipide bei der Organisation des T-Zellrezeptors; Dissertation Universität Freiburg (2009). www.freidok.uni-freiburg.de/data/6968
|
