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Dr. Martin Holzer

Akademischer Rat
Address: Department of Pharmaceutical Technology and Biopharmacy
Hermann-Herder-Strasse 9
D-79104 Freiburg i. Br.
Phone: +49 761 / 203-4908
Fax: +49 761 / 203-6366
eMail: Martin Holzer
Room No.:  03 005 B (3rd floor)

After his graduation in pharmacy (state examination 2003), Dr. Martin Holzer joined the group of Prof. Dr. Rolf Schubert in 2004 and obtained his PhD degree in 2009. In his PhD studies, he established a preparation procedure for a liposomal membran model that features an asymmetrical distribution of the phospholipids between the outer and inner monolayers of the membrane - as it is found in cell membranes. Furthermore, he developed new methods for quantitative lipid analysis by means of high-performance thin layer chromatography and for the preparation of liposomes with a particularly narrow size distribution and defined membrane curvature. In a collaboration with the Max-Planck Institute of Immunobiology and Epigenetics (Freiburg), he demonstrated the important role of membrane lipids in the oligomerisation of the T cell receptor (a membrane-receptor) using a liposomal model.

Since 2013, Dr. Holzer is responsible for the revision of the online resource "Fiedler - Encyclopedia of Excipients".

Assignments in education

Dr. Holzer accompanies the following courses within the Department of Pharmaceutical Technology:

  • Praktikum "Pharmazeutische Technologie" (STEX)
  • Praktikum "Physikalisch-chemische Übungen" (STEX/BSC)
  • Seminar "Biopharmazie einschließlich arzneiformenbezogener Pharmakokinetik" (STEX/BSC)
  • Seminar und Praktikum "Analytik, physikalische Methoden und Qualitätssicherung bei der Herstellung von Arzneimitteln" (BSC)
  • Seminar und Praktikum "Analytik nanopartikulärer Systeme" (MSC)
  • Methodenkurs "Methoden der Arzneimittelherstellung" (MSC)

Scientific methods

  • Asymmetrical flow field-flow fractionation (AF4) with multiangle-light-scattering-, UV/VIS- and fluorescence detectors (in development phase)
  • Free-flow electrophoresis
  • Dynamic light scattering
  • Zeta potential measurements
  • High-performance thin layer chromatography for lipid analysis
  • Size exclusion chromatography
  • HPLC with corona charged aerosol detector
  • Liquid scintillation
  • Ultracentrifugation

Scientific publications

  • De Souza, T.P.; Holzer, M.; Stano, P.; Steiniger, F.; May, S.; Schubert, R.; Fahr, A.; Luisi, P.L.: New insights into the growth and transformation of vesicles: a free-flow electrophoresis study; J. Phys. Chem. B 119: 12212-12223 (2015). dx.doi.org/10.1021/acs.jpcb.5b05057
  • Vögtle, F.N.; Keller, M.; Taskin, A.T.; Horvath, S.E.; Guan, X.L.; Prinz, C.; Opalinska, M.; Zorzin, C., van der Laan, M.; Wenk, M.R.; Schubert, R.; Holzer, M.; Meisinger, C.: The fusogenic lipid phosphatidic acid promotes the biogenesis of mitochondrial outer membrane protein Ugo1; J. Cell Biol. 210: 951-960 (2015). dx.doi.org/10.1083/jcb.201506085
  • Holzer, M.: Fiedler - Encyclopedia of Excipients. Monthly monograph updates 2013-2018; WVG Stuttgart; www.drugbase.de/databases/fiedler.html
  • Molnár, E.; Swamy, M.; Holzer, M.; Beck-García, K.; Worch, R.; Thiele, C.; Guigas, G.; Boye, K.; Luescher, I.F.; Schwille, P.; Schubert, R.; Schamel, W.A.: Cholesterol and sphingomyelin drive ligand-dependent T-cell antigen receptor nanoclustering; J. Biol. Chem. 287: 42664-42674 (2012). dx.doi.org/10.1074/jbc.M112.386045
  • Holzer, M.; Momm, J.; Schubert R.: Lipid transfer mediated by a recombinant pro-sterol carrier protein 2 for the preparation of asymmetrical membrane vesicles requires a narrow vesicle size distribution: a FFE study; Langmuir 26: 4142-4252 (2010). dx.doi.org/10.1021/la903386d
  • Holzer, M.; Burghardt, A.; Schubert, R.: Quantitative high-performance thin layer chromatography determination of liposome components and critical factors influencing the analysis results; J. Liposome Res. 20: 124-133 (2010). dx.doi.org/10.3109/08982100903218884
  • Schneider, S.; Lenz, D.; Holzer, M.; Palme, K.; Süss, R.: Intracellular FRET analysis of lipid/DNA complexes using flow cytometry and fluorescence imaging techniques; J. Control. Rel. 145: 289-296 (2010). dx.doi.org/10.1016/j.jconrel.2010.04.016
  • Holzer, M.; Barnert, S.; Momm, J.; Schubert, R.: Preparative size exclusion chromatography combined with detergent removal as a versatile tool to prepare unilamellar and spherical liposomes of highly uniform size distribution; J. Chrom. A 1216: 5838-5348 (2009). dx.doi.org/10.1016/j.chroma.2009.06.023
  • Holzer, M.: Proteinvermittelter Lipidtransfer zwischen Liposomen zur Herstellung asymmetrischer Modellmembranen und die Rolle der Membranlipide bei der Organisation des T-Zellrezeptors; Dissertation Universität Freiburg (2009). www.freidok.uni-freiburg.de/data/6968


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