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Protein Reconstitution and Protein-Lipid Interactions

Liposomes are particularly suited as a simple model system for investigating the function of membrane proteins depending on a membrane's composition. To this end, enzymes isolated from cell membranes can be reconstituted within liposomal membranes. Collaborating with the group of Professor Dr. Wolfgang Schamel (Institute of Biology III, University of Freiburg, Germany), we successfully reconstituted α/ß-T-cell receptors (TCRs) in model membranes, where upon the influence of different membrane compositions on the TCRs' oligomerization status could be investigated (PhD thesis by Dr. Martin Holzer).

We are currently focusing on the reconstitution of mitochondrial membrane proteins (internal link) as well as on proteins that are suitable to generate asymmetrical membrane states (internal link)..

Polymers coupled to lipidstructures synthesized by the group of Dr. Laura Hartmann at the MPI for Colloids and Interfaces in Potsdam-Golm, Germany, are first vesiculated at this Department and then investigated by IR spectroscopy by the group of Professor Dr. Petra Hellwig at the University of Strasbourg as to their protein-polymer interactions.

 

Molnár E, Swamy M, Holzer M, Beck-García K, Worch R, Thiele C, Guigas G, Boye K, Luescher IF, Schwille P, Schubert R, Schamel WWA: Cholesterol and sphingomyelin drive ligand-independent T-cell antigen receptor nanoclustering; J. Biol. Chem. 287, 42664-74, 2012. [Epub]

Genisyuerek S, Papatheodorou P, Guttenberg G, Schubert R, Benz R, Aktories, K: Structural determinants for membrane insertion, pore formation and translocation of Clostridium difficile toxin B, Mol. Microbiol. 79(6), 1643-54, 2011. [Epub]

 

 

 

 

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