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Instrumental Analyses

High Performance Thin-Layer Chromatography (HPTLC)
In all areas of model-membrane research, it is important to know about the exact membrane composition. This Department therefore developed a quantitative HPTLC method that allows for the simultaneous determination of the most important phospholipids (i.e., phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, cardiolipin, and lyso-phosphatidylcholine) as well as of cholesterol. While developing such methods, we studied fundamental issues of the analysis of phospholipids by HPTLC. These included the stability of phospholipids in the course of analysis as well as the influence of the room humidity on chromatography (PhD thesis by Dr. Martin Holzer, Master thesis by Alexander Burghardt).

High Performance Liquid Chromatography (HPLC) with the Corona Charged Aerosol Detector (CCAD)
In addition to analyzing membrane compositions, we are dealing with analytical questions as to the efficiency of encapsulation processes (hydrophilic drugs in theaqueous interior space of liposomal systems) or of membrane embedding processes (hydrophobic drugs or anchor structures within liposomal membranes). To this end, various HPLC methods are being developed. Moreover, a Corona Charged Aerosol Detector (CCAD) is employed for the highly sensitive detection of compounds that are not UV-active (e.g., lipid-based anchorstructures or pegylatedlipids).

 

Holzer M, Burghardt A, Schubert R: Quantitative high-performance thin layer chromatography determination of common liposome components and critical parameters influencing the analysis results, J. Liposome Res. 20, 124-133, 2010. [Epub]

Schönherr C, Touchene S, Wilser G, Peschka-Süss R, Francese G: Simple and precise detection of lipid compounds present within liposomal formulations using a charged aerosol detector, J. Chromatogr. A 1216, 781-786, 2009. [Epub]

 

 

 

 

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